Towards diagnostic metagenomics of Campylobacter in fecal samples
نویسندگان
چکیده
منابع مشابه
Surveillance of Foodborne Pathogens: Towards Diagnostic Metagenomics of Fecal Samples
Diagnostic metagenomics is a rapidly evolving laboratory tool for culture-independent tracing of foodborne pathogens. The method has the potential to become a generic platform for detection of most pathogens and many sample types. Today, however, it is still at an early and experimental stage. Studies show that metagenomic methods, from sample storage and DNA extraction to library preparation a...
متن کاملDetection of cytolethal distending toxin (cdt) genes of Campylobacter Jejuni and Coli in fecal samples of pet birds in Iran
BACKGROUND: Campylobacteriosis is one of the most commonbacterial intestinal disorders of humans in many countries.A wide range of domestic and wild bird species have been identifiedas natural reservoirs. OBJECTIVES: The aim of this studywas to determine the Campylobacter spp from pet birds andscreen the determined Campylobacters for presence of virulencecytolethal distending toxin (cdt) genes....
متن کاملViral metagenomics of fecal samples from non-human primates revealed human astrovirus in a chimpanzee, China
BACKGROUND Human astroviruses (HAstVs) are commonly identified worldwide as important aetiological agents of acute gastroenteritis in all age groups. More and more evidences challenged the paradigm that AstV infections are species-specific. Yet to date, AstVs associated with human infections have not been detected in any animal hosts. RESULTS Viral metagenomics methods were used to detect vir...
متن کاملDetection of Campylobacter spp. in chicken fecal samples by real-time PCR.
A real-time PCR assay for detecting thermophilic Campylobacter spp. directly in chicken feces has been developed. DNA was isolated from fecal material by using magnetic beads followed by PCR with a prealiquoted PCR mixture, which had been stored at -18 degrees C. Campylobacter could be detected in less than 4 h, with a detection limit of 100 to 150 CFU/ml, in a fecal suspension. A bacterial int...
متن کاملdetection of cytolethal distending toxin (cdt) genes of campylobacter jejuni and coli in fecal samples of pet birds in iran
background: campylobacteriosis is one of the most commonbacterial intestinal disorders of humans in many countries.a wide range of domestic and wild bird species have been identifiedas natural reservoirs. objectives: the aim of this studywas to determine the campylobacter spp from pet birds andscreen the determined campylobacters for presence of virulencecytolethal distending toxin (cdt) genes....
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ژورنال
عنوان ژورنال: BMC Microbiology
سال: 2017
ISSN: 1471-2180
DOI: 10.1186/s12866-017-1041-3